Sodium Dodecyl Sulphate-Sodium Chloride Extraction of Penicillium stoloniferum Mycelium for Viral Double-stranded RNA
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منابع مشابه
Sodium dodecyl sulphate-sodium chloride extraction of Penicillium stoloniferum mycelium for viral double-stranded RNA.
Incubation of mycelia of a virus-containing strain of Penicillium stoloniferum in a I % sodium dodecyl sulphate-4 % sodium chloride (SDS-NaCl) solution liberated protein and deoxyribonucleic acid within the first hours, whereas ribonucleic acid (RNA) release continued through an extended period. Although transfer RNA (tRNA) was readily solubilized by the treatment, viral doublestranded RNA (dsR...
متن کاملSodium Dodecyl Sulphate-Sodium Chloride Extraction of Penicillium stolonwerum Mycelium for Viral Double-stranded RNA
Incubation of mycelia of a virus-containing strain of Penicillium stoloniferum in a I % sodium dodecyl sulphate-4 % sodium chloride (SDS-NaCl) solution liberated protein and deoxyribonucleic acid within the first hours, whereas ribonucleic acid (RNA) release continued through an extended period. Although transfer RNA (tRNA) was readily solubilized by the treatment, viral doublestranded RNA (dsR...
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Reduced and unreduced lysozyme aggregates formed by formaldehyde cross-linking comprise a set of model compounds for studying the effects of protein conformation on the electrophoretic mobilities of sodium dodecyl sulphate-protein complexes. The reduced aggregates were indistinguisable from normal proteins, but the unreduced aggregates migrated anomalously fast by about 14%. Contrary to expecta...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1974
ISSN: 0022-1287
DOI: 10.1099/00221287-80-2-329